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MCM2 Recombinant antibody, PBS Only (Capture)

MCM2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241968D3

Cat no : 84511-2-PBS

Print datasheet

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

验证数据展示

  • WB analysis using 84511-2-RR (same clone as 84511-2-PBS)

    WB analysis using 84511-2-RR (same clone as 84511-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84511-2-RR (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84511-2-RR (same clone as 84511-2-PBS)

    IF Staining of HeLa using 84511-2-RR (same clone as 84511-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (84511-2-RR, Clone: 241968D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84511-2-RR (same clone as 84511-2-PBS)

    FC experiment of HeLa using 84511-2-RR (same clone as 84511-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM2 Recombinant antibody (84511-2-RR, Clone:241968D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01392-2

    Sandwich ELISA standard curve of MP01392-2

    Sandwich ELISA standard curve of MP01392-2, Human MCM2 Recombinant Matched Antibody Pair - PBS only. 84511-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0798. 84511-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP01392-2

    Sample test of MP01392-2

    Serum of six individual healthy human donors was measured. The MCM2 concentration of detected samples was determined to be 2.29 ng/mL with a range of ND-8.68 ng/mL.

  • Sample test of MP01392-2

    Sample test of MP01392-2

    The mean MCM2 concentration was determined to be 58.85 ng/mL in A549 cell extract based on a 2.70 mg/mL extract load and 56.17 ng/mL in HEK-293 cell extract based on a 3.30 mg/mL extract load.

  • Cytometric bead array standard curve of MP01392-1

    Cytometric bead array standard curve of MP01392-1

    Cytometric bead array standard curve of MP01392-1, MCM2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84511-2-PBS. Detection antibody: 84511-1-PBS. Standard: Ag0798. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01392-2

    Cytometric bead array standard curve of MP01392-2

    Cytometric bead array standard curve of MP01392-2, MCM2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84511-2-PBS. Detection antibody: 84511-3-PBS. Standard: Ag0798. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84511-2-RR

    Affinity and Kinetic Characterization of 84511-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84511-2-RR against Human MCM2 were performed. The affinity constant is 0.453 nM.

Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

产品信息

84511-2-PBS targets MCM2 as part of a matched antibody pair:

MP01392-1: 84511-2-PBS capture and 84511-1-PBS detection (validated in Cytometric bead array)

MP01392-2: 84511-2-PBS capture and 84511-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name minichromosome maintenance complex component 2
Synonyms KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 116-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49736
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.