• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84792-2-RR (same clone as 84792-2-PBS)

    WB analysis using 84792-2-RR (same clone as 84792-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84792-2-RR (MCM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 84792-2-RR (same clone as 84792-2-PBS)

    IF Staining of Raji using 84792-2-RR (same clone as 84792-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using MCM3 antibody (84792-2-RR, Clone: 242103E4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 84792-2-RR (same clone as 84792-2-PBS)

    FC experiment of K-562 using 84792-2-RR (same clone as 84792-2-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Mcm3 Recombinant Antibody (84792-2-RR, Clone:242103E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84792-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01593-1

    Cytometric bead array standard curve of MP01593-1

    Cytometric bead array standard curve of MP01593-1, MCM3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84792-2-PBS. Detection antibody: 84792-3-PBS. Standard: Ag7966. Range: 0.469-60 ng/mL.

MCM3 Recombinant antibody, PBS Only (Capture)

MCM3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84792-2-PBS

产品说明书

CloneNo. 242103E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MCM3 Ready-To-Use IHC Kit. MCM3 primary antibody included.

产品信息

84792-2-PBS targets MCM3 as part of a matched antibody pair:

MP01593-1: 84792-2-PBS capture and 84792-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 MCM3 fusion protein Ag7966 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 minichromosome maintenance complex component 3
别名 P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1
计算分子量 91 kDa
观测分子量 102-105 kDa
GenBank蛋白编号BC001626
基因名称 MCM3
Gene ID (NCBI) 4172
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25205
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).