MCM4 Monoclonal antibody
MCM4 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2H2A1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, A431 cells, HL-60 cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy MCM4 Ready-To-Use IHC Kit. MCM4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67103-1-Ig targets MCM4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MCM4 fusion protein Ag28642 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | minichromosome maintenance complex component 4 |
| Synonyms | CDC21, CDC21 homolog, CDC54, hCdc21, MCM4, P1 CDC21 |
| Calculated Molecular Weight | 863 aa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC031061 |
| Gene Symbol | MCM4 |
| Gene ID (NCBI) | 4173 |
| RRID | AB_2882408 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P33991 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of
two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box
of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCM4 antibody 67103-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Heliyon Co-delivery of siRNA and cisplatin via electrospun Nanofibrous membranes for synergistic treatment of malignant melanoma |
