CoraLite® Plus 488-conjugated MCM4 Monoclonal antibody
MCM4 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H2A1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy MCM4 Ready-To-Use IHC Kit. MCM4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67103-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67103 targets MCM4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | MCM4 fusion protein Ag28642 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | minichromosome maintenance complex component 4 |
| Synonyms | P1 CDC21, hCdc21, EC:3.6.4.12, CDC54, CDC21 |
| Calculated Molecular Weight | 863 aa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC031061 |
| Gene Symbol | MCM4 |
| Gene ID (NCBI) | 4173 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P33991 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of
two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box
of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MCM4 antibody CL488-67103 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
