MCM5 Monoclonal antibody
MCM5 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3B7F8
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, A375 cells, HeLa cells, ROS1728 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MCM5 Ready-To-Use IHC Kit. MCM5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67049-1-Ig targets MCM5 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MCM5 fusion protein Ag28704 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | minichromosome maintenance complex component 5 |
| Synonyms | CDC46, CDC46 homolog, MCM5, P1 CDC46 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 82-88 kDa |
| GenBank Accession Number | BC000142 |
| Gene Symbol | MCM5 |
| Gene ID (NCBI) | 4174 |
| RRID | AB_2882362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P33992 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCM5 antibody 67049-1-Ig | Download protocol |
| IF protocol for MCM5 antibody 67049-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5. | ||
Nat Cancer Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis. |
