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CoraLite®594-conjugated MCM5 Monoclonal antibody

MCM5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B7F8

Cat no : CL594-67049

Print datasheet

Synonyms

CDC46, CDC46 homolog, MCM5, P1 CDC46

验证数据展示

  • FC experiment of MCF-7 using CL594-67049

    FC experiment of MCF-7 using CL594-67049

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MCM5 (CL594-67049, Clone:3B7F8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MCM5 Ready-To-Use IHC Kit. MCM5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67049-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67049 targets MCM5 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen MCM5 fusion protein Ag28704 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name minichromosome maintenance complex component 5
Synonyms CDC46, CDC46 homolog, MCM5, P1 CDC46
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 82-88 kDa
GenBank Accession NumberBC000142
Gene Symbol MCM5
Gene ID (NCBI) 4174
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP33992
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

实验方案

Product Specific Protocols
FC protocol for CL594 MCM5 antibody CL594-67049Download protocol
Standard Protocols
Click here to view our Standard Protocols