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验证数据展示

  • FC experiment of MCF-7 using CL594-67049

    FC experiment of MCF-7 using CL594-67049

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MCM5 (CL594-67049, Clone:3B7F8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated MCM5 Monoclonal antibody

MCM5 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67049

产品说明书

CloneNo. 3B7F8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

CDC46, CDC46 homolog, MCM5, P1 CDC46

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MCM5 Ready-To-Use IHC Kit. MCM5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67049-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67049 targets MCM5 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 MCM5 fusion protein Ag28704 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 5
别名 CDC46, CDC46 homolog, MCM5, P1 CDC46
计算分子量 82 kDa
观测分子量 82-88 kDa
GenBank蛋白编号BC000142
基因名称 MCM5
Gene ID (NCBI) 4174
RRIDAB_3084743
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP33992
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

实验方案

Product Specific Protocols
FC protocol for CL594 MCM5 antibody CL594-67049Download protocol
Standard Protocols
Click here to view our Standard Protocols