• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Mouse MCP-1 Rabbit Recombinant Antibody

MCP-1 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230483A9

Cat No : 98028-1-RR

Print datasheet

Synonyms

Ccl2, MCP1, MCP-1, 230483A9, C-C motif chemokine 2, CCL 2, CCL-2, chemokine (C C motif) ligand 2, Chemokine (CC motif) ligand 2, MCP 1

验证数据展示

  • FC experiment of RAW 264.7 using 98028-1-RR

    FC experiment of RAW 264.7 using 98028-1-RR

    1x10^6 unstimulated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of RAW 264.7 using 98028-1-RR

    FC experiment of RAW 264.7 using 98028-1-RR

    1x10^6 unstimulated (black) or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse MCP-1 Rabbit Recombinant Antibody (98028-1-RR, Clone: 230483A9) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inLPS and Brefeldin A treated RAW 264.7 cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98028-1-RR is MCP-1 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms Ccl2, MCP1, MCP-1, 230483A9, C-C motif chemokine 2, CCL 2, CCL-2, chemokine (C C motif) ligand 2, Chemokine (CC motif) ligand 2, MCP 1
GenBank Accession NumberNM-011333
Gene Symbol MCP-1
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10148
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

实验方案

Product Specific Protocols
FC protocol for MCP-1 antibody 98028-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols