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CoraLite® Plus 647 Anti-Mouse MCP-1/CCL2 Rabbit Recombinant Antibody

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230483A9

Cat no : CL647-98028

Print datasheet

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

验证数据展示

  • FC experiment of RAW 264.7 using CL647-98028

    FC experiment of RAW 264.7 using CL647-98028

    1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of RAW 264.7 using CL647-98028

    FC experiment of RAW 264.7 using CL647-98028

    1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9)(red) or Isotype Control (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL647-98028, Clone:230483A9) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated RAW 264.7 cells
For other applications, we recommend the unconjugated version of this antibody, 98028-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-98028 is MCP-1/CCL2 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2
GenBank Accession NumberNM-011333
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10148
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MCP-1/CCL2 antibody CL647-98028Download protocol
Standard Protocols
Click here to view our Standard Protocols