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MDC1 Recombinant antibody, PBS Only (Detector)

MDC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240469D5

Cat no : 83496-1-PBS

Print datasheet

Synonyms

NFBD1, NFBD 1, MDC 1, 240469D5

验证数据展示

  • WB analysis of human testis using 83496-1-RR (same clone as 83496-1-PBS)

    WB analysis of human testis using 83496-1-RR (same clone as 83496-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83496-1-PBS in a different storage buffer formulation.

  • WB analysis using 83496-1-RR (same clone as 83496-1-PBS)

    WB analysis using 83496-1-RR (same clone as 83496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83496-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83496-1-RR (same clone as 83496-1-PBS)

    IF Staining of HeLa using 83496-1-RR (same clone as 83496-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MDC1 antibody (83496-1-RR, Clone: 240469D5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83496-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00487-1

    Sandwich ELISA standard curve of MP00487-1

    Sandwich ELISA standard curve of MP00487-1, Human MDC1 Recombinant Matched Antibody Pair - PBS only. 83496-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20135. 83496-1-PBS was HRP conjugated as the detection antibody. Range: 39.06-2500 pg/mL

  • Sample test of MP00487-1

    Sample test of MP00487-1

    L02 and Hela cell lysate were measured. The human MDC1 concentration of detected samples was determined to be 42.2 ng/mL (based on a 2.6 mg/mL extract load) in L02 cell lysate and 7.6 ng/mL (based on a 1.2 mg/mL extract load) in Hela cell lysate.

  • Cytometric bead array standard curve of MP00487-1

    Cytometric bead array standard curve of MP00487-1

    Cytometric bead array standard curve of MP00487-1, MDC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83496-5-PBS. Detection antibody: 83496-1-PBS. Standard: Ag20135. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00487-3

    Cytometric bead array standard curve of MP00487-3

    Cytometric bead array standard curve of MP00487-3, MDC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83496-2-PBS. Detection antibody: 83496-1-PBS. Standard: Ag20135. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83496-1-RR

    Affinity and Kinetic Characterization of 83496-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83496-1-RR against Human MDC1 were performed. The affinity constant is 0.346 nM.

产品信息

83496-1-PBS targets MDC1 as part of a matched antibody pair:

MP00487-1: 83496-5-PBS capture and 83496-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00487-3: 83496-2-PBS capture and 83496-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen MDC1 fusion protein Ag20135 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mediator of DNA damage checkpoint 1
Synonyms NFBD1, NFBD 1, MDC 1, 240469D5
Calculated Molecular Weight 2089 aa, 227 kDa
GenBank Accession NumberBC152556
Gene Symbol MDC1
Gene ID (NCBI) 9656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14676
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C