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验证数据展示

  • WB analysis of human testis using 83496-1-RR

    WB analysis of human testis using 83496-1-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83496-1-RR

    WB analysis using 83496-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83496-1-RR

    IF Staining of HeLa using 83496-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MDC1 antibody (83496-1-RR, Clone: 240469D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83496-1-RR

    Affinity and Kinetic Characterization of 83496-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83496-1-RR against Human MDC1 were performed. The affinity constant is 0.346 nM.

MDC1 Recombinant antibody

MDC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83496-1-RR

产品说明书

CloneNo. 240469D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

NFBD1, NFBD 1, MDC 1, 240469D5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, HeLa cells, HEK-293 cells, MCF-7 cells, K-562 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MDC1 Ready-To-Use IHC Kit. MDC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83496-1-RR targets MDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MDC1 fusion protein Ag20135 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator of DNA damage checkpoint 1
别名 NFBD1, NFBD 1, MDC 1, 240469D5
计算分子量 2089 aa, 227 kDa
观测分子量260-320 kDa
GenBank蛋白编号BC152556
基因名称 MDC1
Gene ID (NCBI) 9656
RRIDAB_3671123
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14676
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

实验方案

Product Specific Protocols
WB protocol for MDC1 antibody 83496-1-RRDownload protocol
IF protocol for MDC1 antibody 83496-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols