验证数据展示
经过测试的应用
Positive WB detected in | human testis tissue, HeLa cells, HEK-293 cells, MCF-7 cells, K-562 cells, PC-3 cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83496-1-RR targets MDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | MDC1 fusion protein Ag20135 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | mediator of DNA damage checkpoint 1 |
Synonyms | NFBD1, NFBD 1, MDC 1, 240469D5 |
Calculated Molecular Weight | 2089 aa, 227 kDa |
Observed Molecular Weight | 260-320 kDa |
GenBank Accession Number | BC152556 |
Gene Symbol | MDC1 |
Gene ID (NCBI) | 9656 |
RRID | AB_3671123 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14676 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.
实验方案
Product Specific Protocols | |
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WB protocol for MDC1 antibody 83496-1-RR | Download protocol |
IF protocol for MDC1 antibody 83496-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |