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验证数据展示

  • IF Staining of HeLa using CL488-83496

    IF Staining of HeLa using CL488-83496

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MDC1 antibody (CL488-83496, Clone: 240469D5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83496

    FC experiment of HeLa using CL488-83496

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MDC1 Recombinant antibody (CL488-83496, Clone:240469D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated MDC1 Recombinant antibody

MDC1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83496

Print datasheet

CloneNo. 240469D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

NFBD1, NFBD 1, MDC 1, 240469D5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MDC1 Ready-To-Use IHC Kit. MDC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83496-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83496 targets MDC1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen MDC1 fusion protein Ag20135 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mediator of DNA damage checkpoint 1
Synonyms NFBD1, NFBD 1, MDC 1, 240469D5
Calculated Molecular Weight 2089 aa, 227 kDa
Observed Molecular Weight260-320 kDa
GenBank Accession NumberBC152556
Gene Symbol MDC1
Gene ID (NCBI) 9656
RRIDAB_3673259
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14676
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MDC1 antibody CL488-83496Download protocol
FC protocol for CL Plus 488 MDC1 antibody CL488-83496Download protocol
Standard Protocols
Click here to view our Standard Protocols