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验证数据展示

  • WB analysis using 83141-2-RR

    WB analysis using 83141-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83141-2-RR (MDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83141-2-RR

    WB analysis of HEK-293 using 83141-2-RR

    WB result of MDM1 antibody (83141-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM1 transfected HEK-293 cells.

  • IHC staining of mouse testis using 83141-2-RR

    IHC staining of mouse testis using 83141-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83141-2-RR (MDM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83141-2-RR

    Affinity and Kinetic Characterization of 83141-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83141-2-RR against Human MDM1 were performed. The affinity constant is 3.50 nM.

  • Featured Product
  • KD/KO验证

MDM1 Recombinant antibody

MDM1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83141-2-RR

产品说明书

CloneNo. 230425E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Nuclear protein MDM1, 230425E10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Raji cells, PC-3 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83141-2-RR targets MDM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MDM1 fusion protein Ag10541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Mdm1 nuclear protein homolog (mouse)
别名 Nuclear protein MDM1, 230425E10
计算分子量 81 kDa
观测分子量102 kDa
GenBank蛋白编号BC028355
基因名称 MDM1
Gene ID (NCBI) 56890
RRIDAB_3670841
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TC05
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)

实验方案

Product Specific Protocols
WB protocol for MDM1 antibody 83141-2-RRDownload protocol
IHC protocol for MDM1 antibody 83141-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols