验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 83141-2-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83141-2 targets MDM1 in WB applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | MDM1 fusion protein Ag10541 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Mdm1 nuclear protein homolog (mouse) |
| 别名 | |
| 计算分子量 | 81 kDa |
| 观测分子量 | 102 kDa |
| GenBank蛋白编号 | BC028355 |
| 基因名称 | MDM1 |
| Gene ID (NCBI) | 56890 |
| RRID | AB_3673772 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8TC05 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 MDM1 antibody CL750-83141-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |