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MELK Recombinant antibody, PBS Only (Capture/Detector)

MELK Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230106A4

Cat no : 82893-2-PBS

Print datasheet

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

验证数据展示

  • WB analysis using 82893-2-RR (same clone as 82893-2-PBS)

    WB analysis using 82893-2-RR (same clone as 82893-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82893-2-RR (MELK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00075-4

    Sandwich ELISA standard curve of MP00075-4

    Sandwich ELISA standard curve of MP00075-4, Human MELK Recombinant Matched Antibody Pair - PBS only. 82893-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1897. 82893-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP00075-4

    Sample test of MP00075-4

    NCCIT and K-562 cell lysate were measured. The human MELK concentration of detected samples was determined to be 15.9 ng/mL (based on a 4.5 mg/mL extract load) in NCCIT cell lysate and 16.4 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate.

  • IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

    IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

    IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00075-1

    Cytometric bead array standard curve of MP00075-1

    Cytometric bead array standard curve of MP00075-1, MELK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82893-2-PBS. Detection antibody: 82893-3-PBS. Standard: Ag1897. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP00075-2

    Cytometric bead array standard curve of MP00075-2

    Cytometric bead array standard curve of MP00075-2, MELK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82893-4-PBS. Detection antibody: 82893-2-PBS. Standard: Ag1897. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 82893-2-RR

    Affinity and Kinetic Characterization of 82893-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-2-RR against Human MELK were performed. The affinity constant is below 1 pM.

82893-2-RR

产品信息

82893-2-PBS targets MELK as part of a matched antibody pair:

MP00075-1: 82893-2-PBS capture and 82893-3-PBS detection (validated in Cytometric bead array)

MP00075-2: 82893-4-PBS capture and 82893-2-PBS detection (validated in Cytometric bead array)

MP00075-4: 82893-2-PBS capture and 82893-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen MELK fusion protein Ag1897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name maternal embryonic leucine zipper kinase
Synonyms KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2
Calculated Molecular Weight 651 aa, 75 kDa
Observed Molecular Weight50-70 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14680
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.