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验证数据展示

  • IF Staining of HeLa using CL488-84209-5

    IF Staining of HeLa using CL488-84209-5

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 METTL6 antibody (CL488-84209-5, Clone: 241482F9 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated METTL6 Recombinant antibody

METTL6 Recombinant Antibody for IF/ICC
Cat No. CL488-84209-5
CloneNo. 241482F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 84209-5-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84209-5 targets METTL6 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen METTL6 fusion protein Ag9714 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methyltransferase like 6
Synonyms
Calculated Molecular Weight 255 aa, 30 kDa
GenBank Accession NumberBC022400
Gene Symbol METTL6
Gene ID (NCBI) 131965
RRIDAB_3673348
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TCB7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 METTL6 antibody CL488-84209-5Download protocol
Standard Protocols
Click here to view our Standard Protocols