CoraLite® Plus 488-conjugated METTL6 Recombinant antibody
METTL6 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
241482F9
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 84209-5-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84209-5 targets METTL6 in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | METTL6 fusion protein Ag9714 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | methyltransferase like 6 |
| Synonyms | |
| Calculated Molecular Weight | 255 aa, 30 kDa |
| GenBank Accession Number | BC022400 |
| Gene Symbol | METTL6 |
| Gene ID (NCBI) | 131965 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8TCB7 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 METTL6 antibody CL488-84209-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |