CoraLite® Plus 750 Anti-Human MICA/MICB (6D4)
MICA/MICB Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
6D4
验证数据展示
经过测试的应用
| Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 65161-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65161 targets MICA/MICB in FC applications and shows reactivity with Human samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Human |
| Immunogen | Transfected C1R cells expressing MICA 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | MHC class I polypeptide-related sequence A |
| Synonyms | FLJ60820, MIC A, MICA, PERB11.1 |
| Calculated Molecular Weight | 383 aa, 43 kDa |
| GenBank Accession Number | BC016929 |
| Gene Symbol | MICA |
| Gene ID (NCBI) | 100507436 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | Q29983 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 MICA/MICB antibody CL750-65161 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
