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验证数据展示

  • WB analysis using 83242-3-RR (same clone as 83242-3-PBS)

    WB analysis using 83242-3-RR (same clone as 83242-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83242-3-RR (MLLT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    WB analysis of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    WB result of MLLT1 antibody (83242-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLLT1 transfected HeLa cells. This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    IF Staining of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (83242-3-RR, Clone: 230519E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00158-2

    Sandwich ELISA standard curve of MP00158-2

    Sandwich ELISA standard curve of MP00158-2, Human MLLT1 Recombinant Matched Antibody Pair - PBS only. 83242-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34210. 83242-4-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL

  • Sample test of MP00158-2

    Sample test of MP00158-2

    Hela and U-251 cell lysates were measured. The human MLLT1 concentration of detected samples was determined to be 16384.9 pg/mL (based on a 6.4 mg/mL extract load) in Hela cell lysate and 14242.8 pg/mL (based on a 6.4 mg/mL extract load) in U-251 cell lysate.

  • FC experiment of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    FC experiment of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MLLT1 Recombinant antibody (83242-3-RR, Clone:230519E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00158-1

    Cytometric bead array standard curve of MP00158-1

    Cytometric bead array standard curve of MP00158-1, MLLT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83242-3-PBS. Detection antibody: 83242-2-PBS. Standard: Ag34210. Range: 1.25-40 ng/mL.

  • Cytometric bead array standard curve of MP00158-2

    Cytometric bead array standard curve of MP00158-2

    Cytometric bead array standard curve of MP00158-2, MLLT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83242-3-PBS. Detection antibody: 83242-4-PBS. Standard: Ag34210. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83242-3-RR

    Affinity and Kinetic Characterization of 83242-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83242-3-RR against Human MLLT1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

MLLT1 Recombinant antibody, PBS Only (Capture)

MLLT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83242-3-PBS

产品说明书

CloneNo. 230519E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

230519E7, ENL, LTG19, MLLT 1, Protein ENL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83242-3-PBS targets MLLT1 as part of a matched antibody pair:

MP00158-1: 83242-3-PBS capture and 83242-2-PBS detection (validated in Cytometric bead array)

MP00158-2: 83242-3-PBS capture and 83242-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 MLLT1 fusion protein Ag34210 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
别名 230519E7, ENL, LTG19, MLLT 1, Protein ENL
计算分子量62 kDa
观测分子量75 kDa
GenBank蛋白编号BC148806
基因名称 MLLT1
Gene ID (NCBI) 4298
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ03111
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C