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验证数据展示

  • WB analysis using 83242-3-RR (same clone as 83242-3-PBS)

    WB analysis using 83242-3-RR (same clone as 83242-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83242-3-RR (MLLT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    IF Staining of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (83242-3-RR, Clone: 230519E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00158-2

    Sandwich ELISA standard curve of MP00158-2

    Sandwich ELISA standard curve of MP00158-2, Human MLLT1 Recombinant Matched Antibody Pair - PBS only. 83242-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34210. 83242-4-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL

  • Sample test of MP00158-2

    Sample test of MP00158-2

    Hela and U-251 cell lysates were measured. The human MLLT1 concentration of detected samples was determined to be 16384.9 pg/mL (based on a 6.4 mg/mL extract load) in Hela cell lysate and 14242.8 pg/mL (based on a 6.4 mg/mL extract load) in U-251 cell lysate.

  • FC experiment of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    FC experiment of HeLa using 83242-3-RR (same clone as 83242-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MLLT1 Recombinant antibody (83242-3-RR, Clone:230519E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83242-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00158-1

    Cytometric bead array standard curve of MP00158-1

    Cytometric bead array standard curve of MP00158-1, MLLT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83242-3-PBS. Detection antibody: 83242-2-PBS. Standard: Ag34210. Range: 1.25-40 ng/mL.

  • Cytometric bead array standard curve of MP00158-2

    Cytometric bead array standard curve of MP00158-2

    Cytometric bead array standard curve of MP00158-2, MLLT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83242-3-PBS. Detection antibody: 83242-4-PBS. Standard: Ag34210. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83242-3-RR

    Affinity and Kinetic Characterization of 83242-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83242-3-RR against Human MLLT1 were performed. The affinity constant is below 1 pM.

MLLT1 Recombinant antibody, PBS Only (Capture)

MLLT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83242-3-PBS
CloneNo. 230519E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

YEATS1, Protein ENL, MLLT 1, LTG19, ENL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83242-3-PBS targets MLLT1 as part of a matched antibody pair:

MP00158-1: 83242-3-PBS capture and 83242-2-PBS detection (validated in Cytometric bead array)

MP00158-2: 83242-3-PBS capture and 83242-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen MLLT1 fusion protein Ag34210 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
Synonyms YEATS1, Protein ENL, MLLT 1, LTG19, ENL
Calculated Molecular Weight62 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC148806
Gene Symbol MLLT1
Gene ID (NCBI) 4298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03111
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C