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验证数据展示

  • WB analysis using 83242-3-RR

    WB analysis using 83242-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83242-3-RR (MLLT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83242-3-RR

    IF Staining of HeLa using 83242-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (83242-3-RR, Clone: 230519E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83242-3-RR

    FC experiment of HeLa using 83242-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MLLT1 Recombinant antibody (83242-3-RR, Clone:230519E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83242-3-RR

    Affinity and Kinetic Characterization of 83242-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83242-3-RR against Human MLLT1 were performed. The affinity constant is below 1 pM.

MLLT1 Recombinant antibody

MLLT1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83242-3-RR
CloneNo. 230519E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

YEATS1, Protein ENL, MLLT 1, LTG19, ENL

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83242-3-RR targets MLLT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MLLT1 fusion protein Ag34210 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
Synonyms YEATS1, Protein ENL, MLLT 1, LTG19, ENL
Calculated Molecular Weight62 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC148806
Gene Symbol MLLT1
Gene ID (NCBI) 4298
RRIDAB_3670918
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLLT1 antibody 83242-3-RRDownload protocol
IF protocol for MLLT1 antibody 83242-3-RRDownload protocol
FC protocol for MLLT1 antibody 83242-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols