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  • Featured Product
  • KD/KO Validated

MLPH Polyclonal antibody, PBS Only

MLPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10338-1-PBS

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Synonyms

验证数据展示

  • WB analysis of A375 using 10338-1-AP (same clone as 10338-1-PBS)

    WB analysis of A375 using 10338-1-AP (same clone as 10338-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 10338-1-AP (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

    IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

    IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10338-1-AP (same clone as 10338-1-PBS)

    IF Staining of HeLa using 10338-1-AP (same clone as 10338-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10338-1-AP (Melanophilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • IP experiment of A375 using 10338-1-AP (same clone as 10338-1-PBS)

    IP experiment of A375 using 10338-1-AP (same clone as 10338-1-PBS)

    IP result of anti-Melanophilin (IP:10338-1-AP, 4ug; Detection:10338-1-AP 1:600) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

产品信息

10338-1-PBS targets MLPH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MLPH fusion protein Ag0316 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanophilin
Synonyms
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa, 80 kDa
GenBank Accession NumberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
RRIDAB_2146104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV36
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.