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MLPH Polyclonal antibody

MLPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10338-1-AP

Print datasheet

Synonyms

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3



经过测试的应用

Positive WB detected inA375 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10338-1-AP targets MLPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MLPH fusion protein Ag0316 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanophilin
Synonyms SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa, 80 kDa
GenBank Accession NumberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
RRIDAB_2146104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

实验方案

Product Specific Protocols
WB protocol for MLPH antibody 10338-1-APDownload protocol
IHC protocol for MLPH antibody 10338-1-APDownload protocol
IF protocol for MLPH antibody 10338-1-APDownload protocol
IP protocol for MLPH antibody 10338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IP

J Allergy Clin Immunol

Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.

Authors - Valentina Cetica
humanWB

Theranostics

A novel function of Prohibitin on melanosome transport in melanocytes.

Authors - Chan Song Jo
  • KD Validated
humanWB,IF,IP

Oncogene

O-GlcNAcylation of melanophilin enhances radiation resistance in glioblastoma via suppressing TRIM21 mediated ubiquitination

Authors - Lei Xu
mouseWB

Elife

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

Authors - Kunli Zhao
mouseWB,IF

Part Fibre Toxicol

Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies.

Authors - Min Mu
humanWB

J Invest Dermatol

Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.

Authors - Van Gele Mireille M