验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, Jurkat cells, MCF-7 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
12359-1-AP targets SYTL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | SYTL2 fusion protein Ag3023 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | synaptotagmin-like 2 |
别名 | CHR11SYT, Exophilin 4, KIAA1597, SGA72M, SLP2, SLP2A, synaptotagmin like 2, Synaptotagmin like protein 2, SYTL2 |
计算分子量 | 934 aa, 105 kDa |
观测分子量 | 39 kDa, 100-105 kDa |
GenBank蛋白编号 | BC015540 |
基因名称 | SYTL2 |
Gene ID (NCBI) | 54843 |
RRID | AB_11182492 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9HCH5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Synaptotagmin-like 2 (SYTL2) is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. SYTL2 contains an N-terminal Slp homology domain (SHD), Rab-binding region, and C-terminal tandem C2 domains, C2A and C2B (PMID: 11327731). It interacts with RAB27A and plays a role in promoting docking of RAB27-bound organelles to the plasma membrane (PMID: 17052176). Alternative splicing of SYTL2 gene results in multiple transcript variants encoding distinct isoforms with calculated molecular weights ranging from 39 kDa to 142 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for SYTL2 antibody 12359-1-AP | Download protocol |
IHC protocol for SYTL2 antibody 12359-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Extracell Vesicles Suppression of PD‐L1 release from small extracellular vesicles promotes systemic anti‐tumor immunity by targeting ORAI1 calcium channels
| ||
Nat Commun ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma |