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验证数据展示

  • WB analysis using 12359-1-AP

    WB analysis using 12359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12359-1-AP (SYTL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12359-1-AP

    IHC staining of human breast cancer using 12359-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12359-1-AP (SYTL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 12359-1-AP

    IHC staining of human breast cancer using 12359-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12359-1-AP (SYTL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

SYTL2 Polyclonal antibody

SYTL2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12359-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CHR11SYT, Exophilin 4, KIAA1597, SGA72M, SLP2, SLP2A, synaptotagmin like 2, Synaptotagmin like protein 2, SYTL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12359-1-AP targets SYTL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SYTL2 fusion protein Ag3023 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin-like 2
Synonyms CHR11SYT, Exophilin 4, KIAA1597, SGA72M, SLP2, SLP2A, synaptotagmin like 2, Synaptotagmin like protein 2, SYTL2
Calculated Molecular Weight 934 aa, 105 kDa
Observed Molecular Weight39 kDa, 100-105 kDa
GenBank Accession NumberBC015540
Gene Symbol SYTL2
Gene ID (NCBI) 54843
RRIDAB_11182492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptotagmin-like 2 (SYTL2) is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. SYTL2 contains an N-terminal Slp homology domain (SHD), Rab-binding region, and C-terminal tandem C2 domains, C2A and C2B (PMID: 11327731). It interacts with RAB27A and plays a role in promoting docking of RAB27-bound organelles to the plasma membrane (PMID: 17052176). Alternative splicing of SYTL2 gene results in multiple transcript variants encoding distinct isoforms with calculated molecular weights ranging from 39 kDa to 142 kDa.

实验方案

Product Specific Protocols
WB protocol for SYTL2 antibody 12359-1-APDownload protocol
IHC protocol for SYTL2 antibody 12359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Suppression of PD‐L1 release from small extracellular vesicles promotes systemic anti‐tumor immunity by targeting ORAI1 calcium channels

Authors - Xi Chen
  • KD Validated
View Article
humanIHC

Nat Commun

ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma

Authors - Miwa Tanaka
View Article