验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, human liver tissue, HeLa cells, L02 cells, K-562 cells, K562, Jurkat cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human malignant melanoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17817-1-AP targets RAB27A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat, pig, canine, chicken |
| 免疫原 | RAB27A fusion protein Ag12136 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB27A, member RAS oncogene family |
| 别名 | Rab-27, RAB27, Rab 27, GTP-binding protein Ram, GTP binding protein Ram |
| 计算分子量 | 221 aa, 25 kDa |
| 观测分子量 | 25-27 kDa |
| GenBank蛋白编号 | BC107680 |
| 基因名称 | RAB27A |
| Gene ID (NCBI) | 5873 |
| RRID | AB_2176728 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51159 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB27A antibody 17817-1-AP | Download protocol |
| IHC protocol for RAB27A antibody 17817-1-AP | Download protocol |
| IP protocol for RAB27A antibody 17817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
J Allergy Clin Immunol A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation. | ||
Nat Commun Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.
| ||
Blood Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells. |
