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CoraLite® Plus 488-conjugated MMAB Polyclonal antibody

MMAB Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11137

Print datasheet

Synonyms

EC:2.5.1.-, Corrinoid adenosyltransferase MMAB, cblB, ATR, ATP:co(I)rrinoid adenosyltransferase MMAB

验证数据展示

  • IF Staining of HeLa using CL488-11137

    IF Staining of HeLa using CL488-11137

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MMAB antibody (CL488-11137) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-11137

    FC experiment of HeLa using CL488-11137

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated MMAB Polyclonal antibody (CL488-11137)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11137-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11137 targets MMAB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen MMAB fusion protein Ag1602 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methylmalonic aciduria (cobalamin deficiency) cblB type
Synonyms EC:2.5.1.-, Corrinoid adenosyltransferase MMAB, cblB, ATR, ATP:co(I)rrinoid adenosyltransferase MMAB
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC011831
Gene Symbol MMAB
Gene ID (NCBI) 326625
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EY8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MMAB antibody CL488-11137Download protocol
FC protocol for CL Plus 488 MMAB antibody CL488-11137Download protocol
Standard Protocols
Click here to view our Standard Protocols