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CoraLite® Plus 488-conjugated MMAB Polyclonal antibody

MMAB Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11137

Print datasheet

Synonyms

ATR, cblB, MMAB

验证数据展示

  • IF Staining of HeLa using CL488-11137

    IF Staining of HeLa using CL488-11137

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MMAB antibody (CL488-11137) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11137-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11137 targets MMAB in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen MMAB fusion protein Ag1602 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methylmalonic aciduria (cobalamin deficiency) cblB type
Synonyms ATR, cblB, MMAB
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC011831
Gene Symbol MMAB
Gene ID (NCBI) 326625
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EY8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MMAB antibody CL488-11137Download protocol
Standard Protocols
Click here to view our Standard Protocols