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验证数据展示

  • Sandwich ELISA standard curve of MP00266-1

    Sandwich ELISA standard curve of MP00266-1

    Sandwich ELISA standard curve of MP00266-1, MMP13 Recombinant Matched Antibody Pair, PBS Only. 83188-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag34892. 83188-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • IF Staining of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

    IF Staining of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (83188-2-RR, Clone: 240060B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83188-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

    FC experiment of MCF-7 using 83188-2-RR (same clone as 83188-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-2-RR, Clone:240060B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83188-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00266-1

    Cytometric bead array standard curve of MP00266-1

    Cytometric bead array standard curve of MP00266-1, MMP13 Recombinant Matched Antibody Pair, PBS Only. PBS Only. Capture antibody: 83188-3-PBS. Detection antibody: 83188-2-PBS. Standard: Ag34892. Range: 5-40 ng/mL.

  • Cytometric bead array standard curve of MP00266-2

    Cytometric bead array standard curve of MP00266-2

    Cytometric bead array standard curve of MP00266-2, MMP13 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83188-1-PBS. Detection antibody: 83188-2-PBS. Standard: Ag34892. Range: 5-40 ng/mL.

  • Affinity and Kinetic Characterization of 83188-2-RR

    Affinity and Kinetic Characterization of 83188-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83188-2-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

MMP13 Recombinant antibody, PBS Only (Detector)

MMP13 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 83188-2-PBS

产品说明书

CloneNo. 240060B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83188-2-PBS targets MMP13 as part of a matched antibody pair:

MP00266-1: 83188-3-PBS capture and 83188-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00266-2: 83188-1-PBS capture and 83188-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原 MMP13 fusion protein Ag34892 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 13
别名 Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3
计算分子量 471 aa, 54 kDa
观测分子量 70 kDa, 54 kDa
GenBank蛋白编号BC074808
基因名称 MMP13
Gene ID (NCBI) 4322
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP45452
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523).