验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83188-2-RR targets MMP13 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | MMP13 fusion protein Ag34892 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | matrix metallopeptidase 13 |
别名 | Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3 |
计算分子量 | 471 aa, 54 kDa |
观测分子量 | 70 kDa, 54 kDa |
GenBank蛋白编号 | BC074808 |
基因名称 | MMP13 |
Gene ID (NCBI) | 4322 |
RRID | AB_3670877 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P45452 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523).
实验方案
Product Specific Protocols | |
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IF protocol for MMP13 antibody 83188-2-RR | Download protocol |
FC protocol for MMP13 antibody 83188-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |