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验证数据展示

  • IF Staining of MCF-7 using CL488-83188-2

    IF Staining of MCF-7 using CL488-83188-2

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 MMP13 antibody (CL488-83188-2, Clone: 240060B10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated MMP13 Recombinant antibody

MMP13 Recombinant Antibody for IF/ICC
Cat No. CL488-83188-2

Print datasheet

CloneNo. 240060B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 83188-2-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83188-2 targets MMP13 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen MMP13 fusion protein Ag34892 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name matrix metallopeptidase 13
Synonyms
Calculated Molecular Weight 471 aa, 54 kDa
Observed Molecular Weight 70 kDa, 54 kDa
GenBank Accession NumberBC074808
Gene Symbol MMP13
Gene ID (NCBI) 4322
RRIDAB_3673217
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45452
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MMP13 antibody CL488-83188-2Download protocol
Standard Protocols
Click here to view our Standard Protocols