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CoraLite® Plus 488-conjugated MORC3 Polyclonal antibody

MORC3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24994

Print datasheet

Synonyms

ZCWCC3, ZCW5, NXP2, Nuclear matrix protein 2, MORC family CW-type zinc finger protein 3

验证数据展示

  • IF Staining of A431 using CL488-24994

    IF Staining of A431 using CL488-24994

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MORC3 antibody (CL488-24994) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of A431 using CL488-24994

    FC experiment of A431 using CL488-24994

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MORC3 Polyclonal antibody (CL488-24994)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 24994-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24994 targets MORC3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen MORC3 fusion protein Ag21825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MORC family CW-type zinc finger 3
Synonyms ZCWCC3, ZCW5, NXP2, Nuclear matrix protein 2, MORC family CW-type zinc finger protein 3
Calculated Molecular Weight 939 aa, 107 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC132731
Gene Symbol MORC3
Gene ID (NCBI) 23515
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14149
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MORC3 antibody CL488-24994Download protocol
FC protocol for CL Plus 488 MORC3 antibody CL488-24994Download protocol
Standard Protocols
Click here to view our Standard Protocols