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验证数据展示

  • WB analysis using 83705-2-RR (same clone as 83705-2-PBS)

    WB analysis using 83705-2-RR (same clone as 83705-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83705-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83705-2-RR (same clone as 83705-2-PBS)

    FC experiment of HeLa using 83705-2-RR (same clone as 83705-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOSC2 Recombinant antibody (83705-2-RR, Clone:240747G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83705-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83705-2-RR (same clone as 83705-2-PBS)

    IHC staining of human stomach cancer using 83705-2-RR (same clone as 83705-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83705-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83705-2-RR (same clone as 83705-2-PBS)

    IHC staining of human stomach cancer using 83705-2-RR (same clone as 83705-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83705-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00646-2

    Cytometric bead array standard curve of MP00646-2

    Cytometric bead array standard curve of MP00646-2, MOSC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83705-2-PBS. Detection antibody: 83705-1-PBS. Standard: Ag20694. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83705-2-RR

    Affinity and Kinetic Characterization of 83705-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83705-2-RR against Human MOSC2 were performed. The affinity constant is 0.473 nM.

MOSC2 Recombinant antibody, PBS Only (Capture)

MOSC2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83705-2-PBS

产品说明书

CloneNo. 240747G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2, EC:1.7.-.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83705-2-PBS targets MOSC2 as part of a matched antibody pair:

MP00646-2: 83705-2-PBS capture and 83705-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MOSC2 fusion protein Ag20694 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MOCO sulphurase C-terminal domain containing 2
别名 Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2, EC:1.7.-.-
计算分子量 335 aa, 38 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC011973
基因名称 MOSC2
Gene ID (NCBI) 54996
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ969Z3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).