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MOSC2 Recombinant antibody

MOSC2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240747G6

Cat no : 83705-2-RR

Print datasheet

Synonyms

Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2, EC:1.7.-.-

验证数据展示

  • WB analysis using 83705-2-RR

    WB analysis using 83705-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83705-2-RR

    IHC staining of human stomach cancer using 83705-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 83705-2-RR

    IHC staining of human stomach cancer using 83705-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83705-2-RR

    Affinity and Kinetic Characterization of 83705-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83705-2-RR against Human MOSC2 were performed. The affinity constant is 0.473 nM.

经过测试的应用

Positive WB detected inmouse liver tissue, HUVEC cells, HEK-293 cells, mouse kidney tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83705-2-RR targets MOSC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MOSC2 fusion protein Ag20694 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MOCO sulphurase C-terminal domain containing 2
Synonyms Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2, EC:1.7.-.-
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC011973
Gene Symbol MOSC2
Gene ID (NCBI) 54996
RRIDAB_3671307
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969Z3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).

实验方案

Product Specific Protocols
WB protocol for MOSC2 antibody 83705-2-RRDownload protocol
IHC protocol for MOSC2 antibody 83705-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols