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验证数据展示

  • WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

  • WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells. This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 81610-1-RR (same clone as 81610-1-PBS)

    IHC staining of human liver using 81610-1-RR (same clone as 81610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPO antibody included
  • IF Staining of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    IF Staining of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

  • FC experiment of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    FC experiment of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    1x10^6 HL-60 cells were intracellularly stained with 0.25 ug MPO Recombinant antibody (81610-1-RR, Clone:3J17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81610-1-RR (same clone as 81610-1-PBS)

    IHC staining of human colon cancer using 81610-1-RR (same clone as 81610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPO antibody included
  • Affinity and Kinetic Characterization of 81610-1-RR

    Affinity and Kinetic Characterization of 81610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81610-1-RR against Human MPO were performed. The affinity constant is 3.87 nM.

  • Featured Product
  • KD/KO验证

MPO Recombinant antibody, PBS Only

MPO Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81610-1-PBS

产品说明书

CloneNo. 3J17

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

3J17, EC:1.11.2.2, myeloperoxidase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

产品信息

81610-1-PBS targets MPO in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 MPO fusion protein Ag17564 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myeloperoxidase
别名 3J17, EC:1.11.2.2, myeloperoxidase
计算分子量 745 aa, 84 kDa
观测分子量 59 kDa, 90 kDa
GenBank蛋白编号BC130476
基因名称 MPO
Gene ID (NCBI) 4353
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05164
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.