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  • Featured Product
  • KD/KO Validated

MPO Recombinant antibody, PBS Only

MPO Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3J17

Cat no : 81610-1-PBS

Print datasheet

Synonyms

myeloperoxidase, EC:1.11.2.2, 3J17

验证数据展示

  • WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

  • WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    WB analysis of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells. This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 81610-1-RR (same clone as 81610-1-PBS)

    IHC staining of human liver using 81610-1-RR (same clone as 81610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MPO antibody included
  • IF Staining of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    IF Staining of HL-60 using 81610-1-RR (same clone as 81610-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81610-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81610-1-RR

    Affinity and Kinetic Characterization of 81610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81610-1-RR against Human MPO were performed. The affinity constant is 3.87 nM.

Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

产品信息

81610-1-PBS targets MPO in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MPO fusion protein Ag17564 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloperoxidase
Synonyms myeloperoxidase, EC:1.11.2.2, 3J17
Calculated Molecular Weight 745 aa, 84 kDa
Observed Molecular Weight 59 kDa, 90 kDa
GenBank Accession NumberBC130476
Gene Symbol MPO
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05164
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.