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  • Featured Product
  • KD/KO Validated

MPO Recombinant antibody

MPO Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3J17

Cat no : 81610-1-RR

Print datasheet

Synonyms

myeloperoxidase, EC:1.11.2.2, 3J17

验证数据展示

  • WB analysis of HL-60 using 81610-1-RR

    WB analysis of HL-60 using 81610-1-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 81610-1-RR

    WB analysis of HL-60 using 81610-1-RR

    WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells.

  • IHC staining of human liver using 81610-1-RR

    IHC staining of human liver using 81610-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MPO antibody included
  • IF Staining of HL-60 using 81610-1-RR

    IF Staining of HL-60 using 81610-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 81610-1-RR

    IHC staining of human colon cancer using 81610-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MPO antibody included
  • Affinity and Kinetic Characterization of 81610-1-RR

    Affinity and Kinetic Characterization of 81610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81610-1-RR against Human MPO were performed. The affinity constant is 3.87 nM.

经过测试的应用

Positive WB detected inHL-60 cells, human saliva
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81610-1-RR targets MPO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MPO fusion protein Ag17564 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloperoxidase
Synonyms myeloperoxidase, EC:1.11.2.2, 3J17
Calculated Molecular Weight 745 aa, 84 kDa
Observed Molecular Weight 59 kDa, 90 kDa
GenBank Accession NumberBC130476
Gene Symbol MPO
Gene ID (NCBI) 4353
RRIDAB_2935565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05164
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MPO antibody 81610-1-RRDownload protocol
IHC protocol for MPO antibody 81610-1-RRDownload protocol
IF protocol for MPO antibody 81610-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols