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  • Featured Product
  • KD/KO Validated

MPO Recombinant antibody

MPO Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3J17

Cat no : 81610-1-RR

Print datasheet

Synonyms

myeloperoxidase, EC:1.11.2.2, 3J17

验证数据展示

  • WB analysis of HL-60 using 81610-1-RR

    WB analysis of HL-60 using 81610-1-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 81610-1-RR

    WB analysis of HL-60 using 81610-1-RR

    WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells.

  • IHC staining of human liver using 81610-1-RR

    IHC staining of human liver using 81610-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MPO antibody included
  • IF Staining of HL-60 using 81610-1-RR

    IF Staining of HL-60 using 81610-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 81610-1-RR

    IHC staining of human colon cancer using 81610-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MPO antibody included
  • Affinity and Kinetic Characterization of 81610-1-RR

    Affinity and Kinetic Characterization of 81610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81610-1-RR against Human MPO were performed. The affinity constant is 3.87 nM.

经过测试的应用

Positive WB detected inHL-60 cells, human saliva
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81610-1-RR targets MPO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MPO fusion protein Ag17564 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myeloperoxidase
Synonyms myeloperoxidase, EC:1.11.2.2, 3J17
Calculated Molecular Weight 745 aa, 84 kDa
Observed Molecular Weight 59 kDa, 90 kDa
GenBank Accession NumberBC130476
Gene Symbol MPO
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05164
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MPO antibody 81610-1-RRDownload protocol
IHC protocol for MPO antibody 81610-1-RRDownload protocol
IF protocol for MPO antibody 81610-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols