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验证数据展示

  • FC experiment of Hela using CL555-14795

    FC experiment of Hela using CL555-14795

    1X10^6 Hela cells were intracellularly stained with 0.8 ug CoraLite® Plus 555 Anti-Human MRPL12 (CL555-14795) (red), or 0.8 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL555-14795

    IF Staining of HepG2 using CL555-14795

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 MRPL12 antibody (CL555-14795) at dilution of 1:200, CL488-phalloidin (green).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 555-conjugated MRPL12 Polyclonal antibody

MRPL12 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-14795

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7, MRPL7/L12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy MRPL12 Ready-To-Use IHC Kit. MRPL12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14795-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14795 targets MRPL12 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL12 fusion protein Ag6504 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L12
别名 5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7, MRPL7/L12
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC002344
基因名称 MRPL12
Gene ID (NCBI) 6182
RRIDAB_2934640
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52815
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 555 MRPL12 antibody CL555-14795Download protocol
Standard Protocols
Click here to view our Standard Protocols