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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated MRPL12 Polyclonal antibody

MRPL12 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-14795

Print datasheet

Synonyms

5c5 2, FLJ60124, L12mt, MRP L12, MRP L31/34, MRPL12, MRPL7, MRPL7/L12, RPML12

验证数据展示

  • FC experiment of Hela using CL555-14795

    FC experiment of Hela using CL555-14795

    1X10^6 Hela cells were intracellularly stained with 0.8 ug CoraLite® Plus 555 Anti-Human MRPL12 (CL555-14795) (red), or 0.8 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHela cells
Positive FC detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy MRPL12 Ready-To-Use IHC Kit. MRPL12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14795-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14795 targets MRPL12 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MRPL12 fusion protein Ag6504 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L12
Synonyms 5c5 2, FLJ60124, L12mt, MRP L12, MRP L31/34, MRPL12, MRPL7, MRPL7/L12, RPML12
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC002344
Gene Symbol MRPL12
Gene ID (NCBI) 6182
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52815
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 MRPL12 antibody CL555-14795Download protocol
Standard Protocols
Click here to view our Standard Protocols