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CoraLite® Plus 647-conjugated MRPL12 Polyclonal antibody
MRPL12 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MRPL12 Ready-To-Use IHC Kit. MRPL12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14795-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-14795 targets MRPL12 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | MRPL12 fusion protein Ag6504 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitochondrial ribosomal protein L12 |
| Synonyms | RPML12, MRPL7/L12, MRPL7, MRP-L12, Large ribosomal subunit protein bL12m |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC002344 |
| Gene Symbol | MRPL12 |
| Gene ID (NCBI) | 6182 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52815 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 MRPL12 antibody CL647-14795 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
