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验证数据展示

  • WB analysis using 68713-1-Ig

    WB analysis using 68713-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68713-1-Ig (MTF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MTF2 Monoclonal antibody

MTF2 Monoclonal Antibody for WB, ELISA
Cat No. 68713-1-Ig

产品说明书

CloneNo. 2E5D5

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Rat, Rabbit, Mouse

应用

WB, ELISA

别名

dJ976O13.2, hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Rabbit testis tissue, Mouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68713-1-Ig targets MTF2 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Rat, Rabbit, Mouse
免疫原 MTF2 fusion protein Ag9384 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 metal response element binding transcription factor 2
别名 dJ976O13.2, hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1
计算分子量 536 aa, 61 kDa
观测分子量55 kDa, 60 kDa, 75 kDa
GenBank蛋白编号BC010013
基因名称 MTF2
Gene ID (NCBI) 22823
RRIDAB_3670406
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y483
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.

实验方案

Product Specific Protocols
WB protocol for MTF2 antibody 68713-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols