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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MTHFD1 Monoclonal antibody

MTHFD1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B1G3

Cat no : CL488-67670

Print datasheet

Synonyms

MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic

验证数据展示

  • IF Staining of HEK-293 using CL488-67670

    IF Staining of HEK-293 using CL488-67670

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 MTHFD1 antibody (CL488-67670, Clone: 2B1G3 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-67670

    FC experiment of HEK-293 using CL488-67670

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MTHFD1 (CL488-67670, Clone:2B1G3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67670-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67670 targets MTHFD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MTHFD1 fusion protein Ag24553 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Synonyms MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC009806
Gene Symbol MTHFD1
Gene ID (NCBI) 4522
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11586
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MTHFD1 antibody CL488-67670Download protocol
FC protocol for CL Plus 488 MTHFD1 antibody CL488-67670Download protocol
Standard Protocols
Click here to view our Standard Protocols