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验证数据展示

  • IF Staining of HEK-293 using CL555-67670

    IF Staining of HEK-293 using CL555-67670

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 MTHFD1 antibody (CL555-67670, Clone: 2B1G3 ) at dilution of 1:200.

  • IF Staining of HEK-293 using CL555-67670

    IF Staining of HEK-293 using CL555-67670

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 MTHFD1 antibody (CL555-67670, Clone: 2B1G3 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated MTHFD1 Monoclonal antibody

MTHFD1 Monoclonal Antibody for IF/ICC
Cat No. CL555-67670

产品说明书

CloneNo. 2B1G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67670-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67670 targets MTHFD1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 MTHFD1 fusion protein Ag24553 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
别名 MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic
计算分子量 101 kDa
观测分子量 101 kDa
GenBank蛋白编号BC009806
基因名称 MTHFD1
Gene ID (NCBI) 4522
RRIDAB_2934685
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP11586
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

实验方案

Product Specific Protocols
IF protocol for CL555 MTHFD1 antibody CL555-67670Download protocol
Standard Protocols
Click here to view our Standard Protocols