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MTHFD1L Monoclonal antibody, PBS Only

MTHFD1L Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Rabbit, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1F2

Cat No : 68321-1-PBS

Print datasheet

Synonyms

dJ292B18.2, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, MTHFD1L

验证数据展示

  • WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

产品信息

68321-1-PBS targets MTHFD1L in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Rabbit, Mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, Rabbit, Mouse
Immunogen MTHFD1L fusion protein Ag9061 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms dJ292B18.2, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, MTHFD1L
Calculated Molecular Weight 978 aa, 106 kDa
Observed Molecular Weight106 kDa
GenBank Accession NumberBC017477
Gene Symbol MTHFD1L
Gene ID (NCBI) 25902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6UB35
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.