• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated MTMR4 Recombinant antibody

MTMR4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241304E11

Cat no : CL488-84328

Print datasheet

Synonyms

KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48

验证数据展示

  • IF Staining of HepG2 using CL488-84328

    IF Staining of HepG2 using CL488-84328

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MTMR4 antibody (CL488-84328, Clone: 241304E11 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 84328-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84328 targets MTMR4 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen MTMR4 fusion protein Ag4310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myotubularin related protein 4
Synonyms KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48
Calculated Molecular Weight 1195 aa, 133 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberBC035609
Gene Symbol MTMR4
Gene ID (NCBI) 9110
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NYA4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MTMR4 antibody CL488-84328Download protocol
Standard Protocols
Click here to view our Standard Protocols