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验证数据展示

  • WB analysis using 84733-5-RR (same clone as 84733-5-PBS)

    WB analysis using 84733-5-RR (same clone as 84733-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84733-5-RR (MXI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84733-5-RR (same clone as 84733-5-PBS)

    FC experiment of HeLa using 84733-5-RR (same clone as 84733-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-5-RR, Clone:242089D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01509-3

    Sandwich ELISA standard curve of MP01509-3

    Sandwich ELISA standard curve of MP01509-3, Human MXI1 Recombinant Matched Antibody Pair - PBS only. 84733-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3024. 84733-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

  • Sample test of MP01509-3

    Sample test of MP01509-3

    The mean MXI1 concentration was determined to be 20.19 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84733-5-RR

    Affinity and Kinetic Characterization of 84733-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84733-5-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

MXI1 Recombinant antibody, PBS Only (Detector)

MXI1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84733-5-PBS

产品说明书

CloneNo. 242089D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

别名

MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84733-5-PBS targets MXI1 as part of a matched antibody pair:

MP01509-3: 84733-1-PBS capture and 84733-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 MXI1 fusion protein Ag3024 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MAX interactor 1
别名 MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11
计算分子量228 aa, 26 kDa
观测分子量 36 kDa
GenBank蛋白编号BC012907
基因名称 MXI1
Gene ID (NCBI) 4601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP50539
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.