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MXI1 Recombinant antibody, PBS Only (Detector)

MXI1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242089D2

Cat no : 84733-5-PBS

Print datasheet

Synonyms

MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11

验证数据展示

  • WB analysis using 84733-5-RR (same clone as 84733-5-PBS)

    WB analysis using 84733-5-RR (same clone as 84733-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84733-5-RR (MXI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84733-5-RR (same clone as 84733-5-PBS)

    FC experiment of HeLa using 84733-5-RR (same clone as 84733-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-5-RR, Clone:242089D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84733-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01509-3

    Sandwich ELISA standard curve of MP01509-3

    Sandwich ELISA standard curve of MP01509-3, Human MXI1 Recombinant Matched Antibody Pair - PBS only. 84733-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3024. 84733-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

  • Sample test of MP01509-3

    Sample test of MP01509-3

    The mean MXI1 concentration was determined to be 20.19 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84733-5-RR

    Affinity and Kinetic Characterization of 84733-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84733-5-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

产品信息

84733-5-PBS targets MXI1 as part of a matched antibody pair:

MP01509-3: 84733-1-PBS capture and 84733-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen MXI1 fusion protein Ag3024 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MAX interactor 1
Synonyms MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11
Calculated Molecular Weight228 aa, 26 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC012907
Gene Symbol MXI1
Gene ID (NCBI) 4601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50539
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.