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验证数据展示

  • WB analysis using 84733-5-RR

    WB analysis using 84733-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84733-5-RR (MXI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84733-5-RR

    FC experiment of HeLa using 84733-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-5-RR, Clone:242089D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84733-5-RR

    Affinity and Kinetic Characterization of 84733-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84733-5-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

MXI1 Recombinant antibody

MXI1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84733-5-RR

产品说明书

CloneNo. 242089D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse brain tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84733-5-RR targets MXI1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 MXI1 fusion protein Ag3024 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MAX interactor 1
别名 MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11
计算分子量228 aa, 26 kDa
观测分子量 36 kDa
GenBank蛋白编号BC012907
基因名称 MXI1
Gene ID (NCBI) 4601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP50539
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.

实验方案

Product Specific Protocols
WB protocol for MXI1 antibody 84733-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols