验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A549 cells, Hela cells, K-562 cells, HL-60 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, HEK-293 cells, U2OS cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80845-1-RR targets c-MYC in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | c-MYC fusion protein Ag1263 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | v-myc myelocytomatosis viral oncogene homolog (avian) |
别名 | MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39 |
计算分子量 | 49 kDa |
观测分子量 | 50-65 kDa |
GenBank蛋白编号 | BC000141 |
基因名称 | MYC |
Gene ID (NCBI) | 4609 |
RRID | AB_2918915 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P01106 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.
实验方案
Product Specific Protocols | |
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WB protocol for c-MYC antibody 80845-1-RR | Download protocol |
IHC protocol for c-MYC antibody 80845-1-RR | Download protocol |
IF protocol for c-MYC antibody 80845-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |