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验证数据展示

  • IF Staining of C2C12 using CL488-10913

    IF Staining of C2C12 using CL488-10913

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CoraLite® Plus 488 MYL3 antibody (CL488-10913) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of C2C12 using CL488-10913

    FC experiment of C2C12 using CL488-10913

    1x10^6 C2C12 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Myl3 Polyclonal Antibody (CL488-10913)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated MYL3 Polyclonal antibody

MYL3 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10913

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Ventricular myosin alkali light chain, Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inC2C12 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL3 Ready-To-Use IHC Kit. MYL3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10913-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10913 targets MYL3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 MYL3 fusion protein Ag1364 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, light chain 3, alkali; ventricular, skeletal, slow
别名 Ventricular myosin alkali light chain, Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb
计算分子量 22 kDa
观测分子量 22-27 kDa
GenBank蛋白编号BC009790
基因名称 MYL3
Gene ID (NCBI) 4634
RRIDAB_3672471
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08590
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MYL3 antibody CL488-10913Download protocol
FC protocol for CL Plus 488 MYL3 antibody CL488-10913Download protocol
Standard Protocols
Click here to view our Standard Protocols