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验证数据展示

  • WB analysis using 84879-5-RR

    WB analysis using 84879-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84879-5-RR

    FC experiment of A431 using 84879-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody (84879-5-RR, Clone:242198F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84879-5-RR

    Affinity and Kinetic Characterization of 84879-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84879-5-RR against Human Methylmalonyl Coenzyme A mutase/MUT were performed. The affinity constant is 1.83 nM.

Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody

Methylmalonyl Coenzyme A mutase/MUT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84879-5-RR

产品说明书

CloneNo. 242198F11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

MUT, Methylmalonyl-CoA isomerase, Methylmalonyl Coenzyme A mutase, Methylmalonyl CoA isomerase, MCM

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, mouse brain tissue, rat liver tissue
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MUT Ready-To-Use IHC Kit. MUT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84879-5-RR targets Methylmalonyl Coenzyme A mutase/MUT in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methylmalonyl Coenzyme A mutase
别名 MUT, Methylmalonyl-CoA isomerase, Methylmalonyl Coenzyme A mutase, Methylmalonyl CoA isomerase, MCM
计算分子量 750 aa, 83 kDa
观测分子量 78 kDa
GenBank蛋白编号BC016282
基因名称 MUT
Gene ID (NCBI) 4594
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP22033
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.

实验方案

Product Specific Protocols
WB protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 84879-5-RRDownload protocol
FC protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 84879-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols