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验证数据展示

  • IF Staining of HeLa using 84815-1-RR (same clone as 84815-1-PBS)

    IF Staining of HeLa using 84815-1-RR (same clone as 84815-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Midkine antibody (84815-1-RR, Clone: 241880H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84815-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84815-1-RR (same clone as 84815-1-PBS)

    FC experiment of HeLa using 84815-1-RR (same clone as 84815-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Midkine Recombinant antibody (84815-1-RR, Clone:241880H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84815-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84815-1-RR

    Affinity and Kinetic Characterization of 84815-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84815-1-RR against Human Midkine were performed. The affinity constant is 35.9 nM.

Midkine Recombinant antibody, PBS Only

Midkine Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84815-1-PBS

产品说明书

CloneNo. 241880H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

MDK, Neurite outgrowth-promoting protein, Neurite outgrowth-promoting factor 2, Amphiregulin-associated protein, 241880H1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MDK Ready-To-Use IHC Kit. MDK primary antibody included.

产品信息

84815-1-PBS targets Midkine in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Midkine fusion protein Ag24547 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 midkine (neurite growth-promoting factor 2)
别名 MDK, Neurite outgrowth-promoting protein, Neurite outgrowth-promoting factor 2, Amphiregulin-associated protein, 241880H1
计算分子量 16 kDa
GenBank蛋白编号BC011704
基因名称 Midkine
Gene ID (NCBI) 4192
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21741
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.