Midkine Recombinant antibody
Midkine Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
241880H1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MDK Ready-To-Use IHC Kit. MDK primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84815-1-RR targets Midkine in IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | Midkine fusion protein Ag24547 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | midkine (neurite growth-promoting factor 2) |
| Synonyms | MDK, Neurite outgrowth-promoting protein, Neurite outgrowth-promoting factor 2, Amphiregulin-associated protein, 241880H1 |
| Calculated Molecular Weight | 16 kDa |
| GenBank Accession Number | BC011704 |
| Gene Symbol | Midkine |
| Gene ID (NCBI) | 4192 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21741 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Midkine antibody 84815-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |