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验证数据展示

  • IF Staining of HeLa using CL488-13365

    IF Staining of HeLa using CL488-13365

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 NAT10 antibody (CL488-13365) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-13365

    FC experiment of NIH/3T3 using CL488-13365

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated NAT10 Polyclonal antibody (CL488-13365)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated NAT10 Polyclonal antibody

NAT10 Polyclonal Antibody for IF/ICC
Cat No. CL488-13365

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

N-acetyltransferase 10, N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 13365-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13365 targets NAT10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 NAT10 fusion protein Ag4184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 10 (GCN5-related)
别名 N-acetyltransferase 10, N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP
计算分子量 1025 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC035558
基因名称 NAT10
Gene ID (NCBI) 55226
RRIDAB_3083938
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0A0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA. (24786082)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NAT10 antibody CL488-13365Download protocol
Standard Protocols
Click here to view our Standard Protocols