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验证数据展示

  • WB analysis using 66088-1-Ig

    WB analysis using 66088-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 66088-1-Ig

    IHC staining of human cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 66088-1-Ig

    IF Staining of HeLa using 66088-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

NCALD Monoclonal antibody

NCALD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66088-1-Ig

产品说明书

CloneNo. 6B4E10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig, rat

应用

WB, IHC, IF/ICC, ELISA

别名

NCALD, neurocalcin delta, neurocalcin δ

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, pig cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman cerebellum tissue, mouse cerebellum tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3200
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66088-1-Ig targets NCALD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, pig, rat
免疫原 NCALD fusion protein Ag18760 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 neurocalcin delta
别名 NCALD, neurocalcin delta, neurocalcin δ
计算分子量 193 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC036098
基因名称 NCALD
Gene ID (NCBI) 83988
RRIDAB_11232229
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61601
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

实验方案

Product Specific Protocols
WB protocol for NCALD antibody 66088-1-IgDownload protocol
IHC protocol for NCALD antibody 66088-1-IgDownload protocol
IF protocol for NCALD antibody 66088-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols