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NCALD Monoclonal antibody

NCALD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6B4E10

Cat no : 66088-1-Ig

Print datasheet

Synonyms

NCALD, neurocalcin delta, neurocalcin δ

验证数据展示

  • WB analysis using 66088-1-Ig

    WB analysis using 66088-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 66088-1-Ig

    IHC staining of human cerebellum using 66088-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 66088-1-Ig

    IF Staining of HeLa using 66088-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, pig cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman cerebellum tissue, mouse cerebellum tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66088-1-Ig targets NCALD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen NCALD fusion protein Ag18760 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name neurocalcin delta
Synonyms NCALD, neurocalcin delta, neurocalcin δ
Calculated Molecular Weight 193 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC036098
Gene Symbol NCALD
Gene ID (NCBI) 83988
RRIDAB_11232229
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61601
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

实验方案

Product Specific Protocols
WB protocol for NCALD antibody 66088-1-IgDownload protocol
IHC protocol for NCALD antibody 66088-1-IgDownload protocol
IF protocol for NCALD antibody 66088-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols