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NCALD Monoclonal antibody, PBS Only

NCALD Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B4E10

Cat no : 66088-1-PBS

Print datasheet

Synonyms

NCALD, neurocalcin delta, neurocalcin δ

验证数据展示

  • WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

    WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

    IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

产品信息

66088-1-PBS targets NCALD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen NCALD fusion protein Ag18760 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name neurocalcin delta
Synonyms NCALD, neurocalcin delta, neurocalcin δ
Calculated Molecular Weight 193 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC036098
Gene Symbol NCALD
Gene ID (NCBI) 83988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61601
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.