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CoraLite® Plus 488-conjugated NCALD Monoclonal antibody

NCALD Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6B4E10

Cat no : CL488-66088

Print datasheet

Synonyms

NCALD, neurocalcin delta

验证数据展示

  • IF Staining of HeLa using CL488-66088

    IF Staining of HeLa using CL488-66088

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NCALD antibody (CL488-66088, Clone: 6B4E10 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66088-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-66088 is NCALD Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, pig
Immunogen NCALD fusion protein Ag18760 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name neurocalcin delta
Synonyms NCALD, neurocalcin delta
Calculated Molecular Weight 193 aa, 22 kDa
GenBank Accession NumberBC036098
Gene Symbol NCALD
Gene ID (NCBI) 83988
RRIDAB_2919285
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61601
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NCALD antibody CL488-66088Download protocol
Standard Protocols
Click here to view our Standard Protocols