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验证数据展示

  • IF Staining of SH-SY5Y using CL532-83365-6

    IF Staining of SH-SY5Y using CL532-83365-6

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®532 NCAM1/CD56 antibody (CL532-83365-6, Clone: 240078C9 ) at dilution of 1:200.

CoraLite®532-conjugated NCAM1/CD56 Recombinant antibody

NCAM1/CD56 Recombinant Antibody for IF/ICC
Cat No. CL532-83365-6

Print datasheet

CloneNo. 240078C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®532

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

库存：1-2周货期

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy NCAM1/CD56 Ready-To-Use IHC Kit. NCAM1/CD56 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83365-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL532-83365-6 targets NCAM1/CD56 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms
Calculated Molecular Weight 95 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
RRIDAB_3673401
Conjugate CoraLite®532 Fluorescent Dye
Excitation/Emission Maxima Wavelengths537 nm / 560 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13591
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

实验方案

Product Specific Protocols
IF protocol for CL532 NCAM1/CD56 antibody CL532-83365-6Download protocol
Standard Protocols
Click here to view our Standard Protocols